Immunohistochemistry and Immunocytochemistry

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. Immunohistochemistry and Immunocytochemistry: Essential Methods is the second edition of Immunohistochemisrty: Methods Express, previously published by Scion Publishing. The book is a comprehensive research guide that describes the methodologies of immunochemistry. Each chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good theoretical information and practical advice, for immediate use at the bench. Subjects covered include, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. This book offers a wealth of knowledge to the novice immunochemist, who wishes to understand fully the theory and practice of immunochemical staining techniques and obtain reliable and reproducible data time and time again. For the experienced immunochemist, it is a comprehensive reference guide, allowing optimization of existing immunochemical staining protocols and a resource to further increase knowledge and understanding.