Molecular Biology Techniques
| Autor: | Sue Carson, Heather B. Miller, Melissa C. Srougi, D. Scott Witherow |
|---|---|
| EAN: | 9780128157756 |
| eBook Format: | ePUB/PDF |
| Sprache: | Englisch |
| Produktart: | eBook |
| Veröffentlichungsdatum: | 05.03.2019 |
| Untertitel: | A Classroom Laboratory Manual |
| Kategorie: | |
| Schlagworte: | Affinity chromatography Agarose gel Annealed oligo cloning BSA Bradford CDNA CRISPR Cell culture Chromas Chromatogram Cleavage Computational Cultures DNase Dilution EGFP E coli Egfp Electrophoresis Escherichia coli Exp Expression |
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Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology-or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. - Presents student-tested labs proven successful in real classroom laboratories - Includes a test bank on a companion website for additional testing and practice - Provides exercises that simulate a cloning project that would be performed in a real research lab - Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
Dr. Carson's area of scientific expertise is in molecular mechanisms of bacterial pathogenesis. She currently serves as the Director of the Master of Microbial Biotechnology Program and as a Fellow in the Office of Faculty Excellence. Prior to this appointment, she led the NC State Quality Enhancement Plan (QEP), focused on faculty development to cultivate students' critical and creative thinking skills across disciplines. During that period, she also acted as Executive Director of Academic Enrichment Programs, overseeing the Office of Undergraduate Research, Fellowship Advising, and the University Honors and Scholars Programs. Dr. Carson spent over ten years leading curriculum development for the NC State Biotechnology Program. She has received multiple awards for teaching excellence and innovation and was a member of the Howard Hughes Science Education Alliance, promoting and implementing inquiry-guided learning and authentic research in undergraduate curricula. She authored three molecular biology lab manuals and has published numerous peer-reviewed papers in the area of course and curriculum development. She has mentored over 100 undergraduate students in research projects and was the PI and Director of the National Science Foundation (NSF)-funded Integrative Plant and Microbial Systems Research Experience for Undergraduates (REU) Program for over a decade. Most recently, she completed a 2-year rotation at the National Science Foundation in the Directorate for Education and Human Resources. Within the Triangle community, she has served on the Board of Directors of the Wake County Beekeeping Association and the Triangle Swing Dance Society.
Dr. Carson's area of scientific expertise is in molecular mechanisms of bacterial pathogenesis. She currently serves as the Director of the Master of Microbial Biotechnology Program and as a Fellow in the Office of Faculty Excellence. Prior to this appointment, she led the NC State Quality Enhancement Plan (QEP), focused on faculty development to cultivate students' critical and creative thinking skills across disciplines. During that period, she also acted as Executive Director of Academic Enrichment Programs, overseeing the Office of Undergraduate Research, Fellowship Advising, and the University Honors and Scholars Programs. Dr. Carson spent over ten years leading curriculum development for the NC State Biotechnology Program. She has received multiple awards for teaching excellence and innovation and was a member of the Howard Hughes Science Education Alliance, promoting and implementing inquiry-guided learning and authentic research in undergraduate curricula. She authored three molecular biology lab manuals and has published numerous peer-reviewed papers in the area of course and curriculum development. She has mentored over 100 undergraduate students in research projects and was the PI and Director of the National Science Foundation (NSF)-funded Integrative Plant and Microbial Systems Research Experience for Undergraduates (REU) Program for over a decade. Most recently, she completed a 2-year rotation at the National Science Foundation in the Directorate for Education and Human Resources. Within the Triangle community, she has served on the Board of Directors of the Wake County Beekeeping Association and the Triangle Swing Dance Society.