Proteomics of Biological Systems

Autor:	Bryan M. Ham
EAN:	9781118137017
eBook Format:	PDF/ePUB
Sprache:	Englisch
Produktart:	eBook
Veröffentlichungsdatum:	26.09.2011
Untertitel:	Protein Phosphorylation Using Mass Spectrometry Techniques
Kategorie:	Technik / Wissen
Schlagworte:	Blast2GO gene ontology mass spectrometry and phosphorylation phosphate (PO4) group phosphoproteomic phosphoproteomic sample preparation phosphorylation protein phosphorylation using MS protein phosphorylation using mass spectrometry proteomic

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Phosphorylation is the addition of a phosphate (PO₄) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.

BRYAN M. HAM, PhD, is a member of the American Society for Mass Spectrometry and the American Chemical Society. He has conducted proteomics and lipidomics research at The Ohio State University and Pacific Northwest National Laboratory in Richland, Washington. He is currently working for the Department of Homeland Security at the U.S. Customs and Border Protection New York Laboratory. He has published numerous research papers in peer-reviewed journals, and is the author of Even Electron Mass Spectrometry with Biomolecule Applications (Wiley).

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